Om P. Srivastava, Ph.D.
Department of Vision Sciences
School of Optometry
Worrell Building, 924 South 18th Street
 University of Alabama at Birmingham
Birmingham, AL 35294-4390

Om Srivastava, PhD

EDUCATION:                               Ph.D. (1976), Study of peroxidase isozymes,

                                                      University of Western Ontario, Canada

                                                                                   

                                                      M.Sc. (1969), Organic Chemistry

                                                      University of Allahabad, India

 

RESEARCH AND PROFESSIONAL EXPERIENCE:

 

1993 - Present   Assistant Professor (1993-1999), Associate Professor (1999-2003),  Professor (2003-present),  Department of Vision Sciences, School of Optometry, University of Alabama at Birmingham, Alabama.

                                    Research:  (1) Role of lens proteinases, their inhibitors and crystallin fragments in human senile cataractogenesis.

            (2) Structure-function of human corneal a-enolase and extracellular matrix proteins in keratoconus and Fuchs’ dystrophy

            (3) Isoflavanoids and oxidative damage to Lens 

 

1985 - 1993        Senior Research Scientist, Missouri Lions Eye Research Foundation, Columbia, Missouri; Assistant Professor, Department of Ophthalmology, University of Missouri-Columbia, Columbia, Missouri.

                                    Research: (1) Role of lens proteinases, their inhibitors and crystallin fragments in human senile cataractogenesis.

                                    (2) Corneal endothelial membrane proteins during aging and Fuch's dystrophy.

 

1984 - 1985        Assistant Professor, Department of Pediatrics, School of Medicine, University of Oklahoma, Oklahoma City, Oklahoma.

                        Research:  Glycosylation of microvillar membrane proteins of small intestine.

 

1982 - 1984        Senior Research Associate, Cancer Research Center/Ellis Fischel State Cancer Center, Columbia, Missouri.

                           Research:  Detection of colon and breast carcinoma using tumor-associated antigens as markers.

 

1979 - 1982        Research Associate, Department of Ophthalmology, University of Missouri, Columbia, Missouri.

                        Research:  Lens proteinases and their inhibitors.

 

1977 - 1979        Research Associate, Department of Biological Sciences, Purdue University, West Lafayette, Indiana.

                           Research:  Spore coat proteins and proteinases in bacilli.

 

MEMBERSHIP IN SCIENTIFIC SOCIETIES:

 

                        The Association for Research in Vision and Ophthalmology

                        International Congress of Eye Research

                        American Society for Biochemistry and Molecular Biology

                        American Association for Advancement of Science (1993-1996)

 

COMMUNITY SERVICES:

 

1985 - 1993     Member of Columbia (Missouri) Evening Lions Club Member

1991 - 1992     President of Columbia Evening Lions Club

1996                Coach for Vestavia under 8 Soccer Club

1997                Coach for Vestavia under 10 Soccer Club

1998-2006       Asst. Scout master, Boy Scouts of America, Troop 312, Birmingham, AL

 

INVITED LECTURES:

 

1988 -              Kirksville College of Osteopathic Medicine, Department of Biochemistry, Kirksville, MO.

1989 -              Alcon Corporation, Fort Worth TX.

1990 -              Oculon Corporation, Seattle, WA.

1991 -              Kirksville College of Osteopathic Medicine, Department of Biochemistry, Kirksville, MO.

1992 -              International Congress of Eye Research, Stresa, Italy.

1994 -              International Congress for Eye Research, New Delhi, India.

                        (Co-Chairperson of the session "Post-translational modification of lens proteins”).

1996-               Department of Ophthalmology, Washington University, St. Louis, MO. (as a visiting scientist)

1999-                              National Eye Institute

1999-                              Department of Ophthalmology, University of Maryland-Baltimore.

2000-                              ARVO Special symposium entitled  "A review of human lens crystallins: The role of post-translational modifications in cataract."

2006-               Center for Aging, University of Alabama at Birmingham, “Potential role of post-translational modifications of crystallins in age-related human  cataract.”

2006-               International Congress for Eye Research, Buenos Aires, Argentina.

                        Talk entitled “Structural and stability properties of truncated human bA3-crystallin” in Lens session #10: Crystallin protein function and biochemistry.

2007:               ARVO Minisymposium- Proteases in lens development and disease, invited talk entitled “Is an Arg-bond hydrolyzing proteinase activity associated with human lens bA3/A1-crystallin?”

2008:               Srivastava O.P. Human age-related cataract: Potential role of post-translational modifications of crystallins, Ohio State University, 2008

2008:               Srivastava O.P. Chaves J.M., Srivastava K. and  Gupta R. Structural and Functional Roles of Deamidation and/or Truncation of N- or C-Termini in Human aA-Crystallin, International Congress of Eye Research, China, 2008.

 

PRESENT RESEARCH INTERESTS:

 

1.         Proteinase, their inhibitors and proteolysis in human lenses during aging and cataractogenesis. Potential role of modified crystallin fragments in cataractogensis.

2.         Structure-function of human corneal 66-kDa big h3 protein and extracellular matrix proteins during aging, Fuchs' dystrophy and keratoconus.

3.         Isoflavanoids and oxidative damage to lens.

 

 

RESEARCH COLLABORATIONS (Past and Present):

           

            Outside UAB:

1.         Dr. Mark Petrash, Professor, Department of Ophthalmology, Washington University, St. Louis, MO.

2.         Dr. R. Nagaraj, Professor, Department of Ophthalmology, Case Western Reserve University, Cleveland, OH, 44106

4.         Dr. R. Pfister, M.D., Brookwood Medical Center, Birmingham, AL.

5.         Dr. Kirstin Lampi, Oregon Health Sciences University, Oregon.

6.         Dr. Paul FitzGerald, University of California, Davis

 

            Within UAB:

1.         Dr. Steve Barnes, Professor, Department of Pharmacology and Toxicology.

2.         Dr. Helen Kim, Department of Pharmacology & Toxicology.

            3.         Dr. Andrea Frost, Department of Pathology

            4.         Dr. James Mobley, Department of Surgery and Urology

           

PROFESSIONAL SERVICES:

 

Service to International Professional Organization:

Member of  ‘Organizational Structure Committee’ for International Society for Eye Research (ISER). [Dr. Gene Anderson as chair, 2003]

 

Service to National Organization:

(i)            National Eye Institute, Visual Sciences A Study Section, Post-doctoral F-32 Grants (1998, 1999).

(ii)     National Eye Institute, RO1 grants in Visual Sciences A Study Section (2000-2006, 2008, Served seven times)

(iii)    National Eye Institute, SBIR grant (2000, 2001, Served two times)

(iv)    Fight for Sight of Prevent Blindness America. Permanent member for a three-year term of the panel to review post-doctoral grants (2001-2003), served every year thereafter (2004-2007) to review, summer student fellowships, post-doctoral and grant-in-aid applications.

 

Reviewer of Grants:

(i)      UAB-Center for Aging

(ii)          Alabama Vision and Imaging Science (ALVIS) Center

(iii)         Retirement Research Foundation Inc., Chicago, IL. (1998)

(iv)        National Eye Institute, Visual Sciences A Study Section, Post-doctoral F-32 Grants (1998, 1999).

(v)          UAB-Center for Aging (1998)       

(vi)        VSRC grants (1998).

(vii)       National Eye Institute, RO1 grants in Visual Sciences A Study Section (2000-2006, Served seven times)

(viii)     National Eye Institute, SBIR grant (2000, 2001, Served two times)

(ix)        Fight for Sight of Prevent Blindness America grants. Permanent member for a three-year term of the panel to review post-doctoral grants (2001-Present)

(x)    Pilot and Bridging Grant –Kansas INBRE (2004)

(xi)    James and Esther King Biomedical Research Program, managed by Florida Department of Health (2007, 2008,2009)           

 

Reviewers for Journals:        

(i)      Investigative Ophthalmology and Visual Science (1997-2006).

(ii)          Experimental Eye Research (1997-2006).

(iii)         Curr. Eye Res. (1998, 1999, 2002, 2004, 2006).

(iv)        J. Biol. Chem. (1999).

(v)          Molecular Vision (2000, 2001, 2003, 2004, 2005).

(vi)        Biochemistry (2002)

(vii)       Biochem Biophys Acta (2004)

(viii)     Proteomics (2005)

(ix)        Journal  of Cellular Biochemistry (2005)

(x)          Molecular and Cellular Biochemistry (2005)

(xi)        Cornea (2005)

(xii)       Molecular Vision (2002-present)

(xiii)     Biopolymers (2006)

 

Departmental and School of Optometry Committees:                   

(i)            Admissions Committee for the VSRC graduate program (1999-present)

(ii)          Vision Sciences Research Center Advisory Committee (1999-present).

(iii)         Academic Review Council, 2001-present

(iv)        Vision Science Faculty Affairs Committee (2005-present)

(v)          School of Optometry Faculty Affairs Committee (2007, 2008)

(vi)        Faculty Search Committee-Vision Sciences (2005)

(vii)       Vision Science committee for a retreat (2004-2005)

(viii)     School of Optometry Faculty Affairs Committee (1999-2002)

(ix)        Member of Self-Study Core Committee for the Accreditation Council on Optometric Education (2001-2002)

(x)          Sub-Committee-VSRC Visiting Scholar Program (1999-2000)

(xi)        NEI Core Grant Advisory Committee (1999-2000)

(xii)       Information Services Committee (1998-1999)

(xiii)     Director of Computer Module (1998-2000).

(xiv)     Optometry Admissions Committee (1995-1998)

(xv)      Continuing Education Committee (1997-1998)

 

Campus-Wide Committees:

(i)            Faculty Policies and Procedures Committee (2002-2008)

(ii)          UAB NCRR K-12 Science Education Program (1997-1999)

(iii)         Alternate senator for School of Optometry in Faculty Senate (1996-1997)

              

Graduate Students Committees:

(i)            O.D./M.S. committee of Richard Kalina (Mentor: Dr. Rod Fullard)

(ii)          O.D./M.S. committee of Deborah Keller (Mentor: Dr. Rod Fullard) 

(iii)         M.S. committee of Earl Primo (Mentor: Dr. Graem Wilson)

(iv)        Ph.D. committee of Quing Chang (Mentor: Dr. David Whikehart)

(v)          Ph.D. committee of Hong Wei Ren (Mentor: Dr. Graem Wilson)

(vi)        Ph.D. committee of John Siegwart (Mentor: Dr. Tom Norton)

(vii)       OD/MS committee of Shilpa (Patel) Register (1999) [Mentor: Dr. David Whikehart)

(viii)     OD/MS committee of Susan Hosmer (2001) [Mentor: Dr. Dennis Pillion]

(ix)        OD/MS committee Alexia Vaughn (2002-2005) [Mentor: Dr. David Whikehart)

(x)          OD/MS committee of Lan Quing  (2002) [Mentor: Dr. Rod Fullard]

(xi)        M.S. committee for Jim Webb (2002) [Mentor: Dr. Rod Fullard]

(xii)       Ph.D. committee of Brandon White (2001) [Mentor: Dr. Steve Pittler]

(xiii)     Ph.D. committee of Kate Shows (2002) [Mentor: Dr. Steve Pittler]

(xiv)     Ph.D. committee (chair) of Chuanqing Ding (2002) [Mentor: Dr. Kent Keyser]

(xv)      Ph.D. committee of Laura Brockway (2002-2004) [Mentor: Dr. Kent Keyser]

(xvi)     Ph.D. committee of John Arnold (2002-2004) [Mentor: Dr. Dennis Pillion, Dept of Pharmacology and Toxicology]

(xvii)   Ph.D. committee of Chidi Ekennakalu (2002-2004) [Mentor: Dr. Rod Fullard]

(xviii)  Ph.D. committee of John Chijuka  (2005) [Mentor: Dr. Paul Gamlin]

(xix)     Ph.D. committee of Veronica Harrington (2005) [Mentor: Om Srivastava]

(xx)      Ph.D committee of  Kai Wang  (2006) [Dept. of Physiolgy and Biophysics]

(xxi)     M.S committee  of Sara McGowan (2007)

(xxii)   Ph.D. committee of Jordan Renna (2007)

(xxiii)  Ph.D. committee of Mauro Chaves (2005-2008)

(xxiv) Ph.D. committee of Chinwe Asomugha (2005-present).

(xxv)   OD/MS committee of  Kayla Vickers (2008)

 

        

         Summer Training of Minority Students:

(i)                  Shannon Milton (1995)

(ii)                David Satyakumar (1996)

(iii)               Millecent Gibson (1996)

(iv)              Brandon Wolf (1997)

(v)        Veronica Harrington (1998,1999)

         (vi)       Janell Thompson (2000)

(xiv)        Shantise McCall (McNair Program, 2001, 2002)

(viii)        Alice Irby (McNair Program, 2004)

(ix)              Catherine  (Minortity program, 2005)

(x)                Joseph Geiger (2006 CORD Summer Science Institute Reseach Intern    Program).

(xi)              LeKisha Lockhart (2007 CORD Summer Science Institute Reseach Intern    Program).

(xii)             Nichele Cantrell (2008 CORD Summer Science Institute Reseach Intern    Program).

 

Mentor of Graduate Students:

(i)         Ernest Sisneros (for OD/MS)

(ii)        Laura Brockway  (during one quarter of rotation for Ph.D.).

(iii)      Deepa Chandrasekaran (Graduated with MS, 2002)

         (iv)       Veronica Harrington (Graduated with Ph.D., 2005)

(v)             Sy Huynh (for OD/MS)

(vi)           Brian Mahalak  (for Ph.D)

(vii)             Kerri Mafia (Dept. of Forensic Sciences,  M.S., Graduated in 2007)

(viii)           David Stella (Dept. of Biochemistry and Molecular Biology, 2006-present, ),

(ix)              Jose M. Chaves (2005-2008, Ph.D., Graduated in 2008)

(x)                Chinwe Asomugha (Ph.D. Student 2005-present).

(xi)              Roy Joseph (Ph.D. Student, 2007-present)

 

Mentor for Post-Doctoral Fellow/Research Associate:

(i)                  Kiran Srivastava (2000-present)

(ii)                Ratna Gupta (2002-present)

(iii)               Jose M. Chaves (2008-present)

(iv)              Sanjay Pradhan (2007)

(v)                Thiagarajan Sambandam  (2000)

(vi)              O .P. Bansal (2000)

(vii)             H.R. Acharya (1996-1997)

    

TEACHING:             

(i)                  Physiology of the Eye (PO 211), Course Master, 1998-present.

(ii)                VSRC core graduate course (Ocular Anatomy, Physiology and Biochemistry,  Vis 744), Course master, 2000-present.

(iii)               VSRC core graduate course, (Ocular Anatomy, Physiology and Biochemistry Vis 745), 2000-present.  

(iv)              Biochemistry of the Eye (PO 710), Delivered about 50% of the lectures in 1998 and several lectures in 2000 and 2001.

(v)                Physiology of the Eye (PO 709), Course Master, 1998

(vi)              Introduction to Graduate Studies in Vision Sciences (PO 700) (1993-1999)

(vii)             Ocular Biochemistry (PO 211, 1995)

 

 

TEACHING EVALUATIONS: 

         Student’s opinions and student evaluations for PO 211 course (Physiology of the Eye) have been very positive for the past seven years. 

 

TEACHING EVALUATIONS BY PEERS:

         I have been nominated for President’s Excellence in Teaching award in 2001, 2002, 2003 (finalist) and 2005. In 2006, I received UAB President’s “Excellence in Teaching Award”.

 

PERFORMANCE OF STUDENTS IN STANDARDIZED TESTS:

         During the period of my teaching as a course master of PO 211 (Physiology of the Eye), the NBEO scores of students have been consistently higher than the nationals average in the Ocular Physiology Section.

 

PUBLICATIONS:

1.                  Srivastava, O.P. and van Huystee, R.B.  Evidence for close association of peroxidase, polyphenol oxidase and IAA oxidase isoenzymes of peanut cell suspension culture.  Can. J. Bot. 51: 2207-2215, 1973.

 

2.                  Srivastava, O.P.  Study of peroxidase isoenzymes isolated from the medium of peanut cells cultures.  Doctoral dissertation.  University of Western Ontario, Canada, 1976.

3.                  Srivastava, O.P. and van Huystee, R.B.  Spectral and molecular properties of peanut peroxidase isoenzymes.  Phytochem. 16: 1657-1659, 1977.

 

4.                  Srivastava, O.P. and van Huystee, R.B.  IAA oxidase and polyphenol oxidase activities of peanut peroxidase isoenzymes.  Phytochem. 16: 1527-1530, 1977.

 

5.                  Srivastava, O.P. and van Huystee, R.B.  Interaction among peroxidase, IAA oxidase and polyphenol oxidase from peanut cells.  Can. J. Bot. 65: 2630-2635, 1977.

 

6.                  Srivastava, O.P. and van Huystee, R.B.  Interaction among peroxidase isoenzymes and phenolics.  Bot. Gaz. 138: 451-464, 1978.

 

7.                  Srivastava, O.P. and Fitz-James, P.C.  Alteration of heat activation of enzymes localized in spore coats of Bacillus cereus.  Can. J. Microbiol. 27: 408-416, 1981.

 

8.                  Srivastava, O.P. and Fitz-James, P.C.  A unique protease from coats of Bacillus cereus.  J. Gen. Microbiol. 126: 463-475, 1981.

 

9.                  Srivastava, O.P and Aronson, A.I.   A new trypsin-like proteinase from sporulating cells of Bacillus subtilis.  Arch. Microbiol. 129: 227-232, 1981.

 

10.              Sastry, K.J., Srivastava, O.P., Miller, J., Fitz-James, P.C. and Aronson, A.I.  Characterization of Bacillus subtilis mutants with a temperature sensitive intracellular proteases.  J. Bacteriol. 153: 511-519, 1983.

 

11.              Srivastava, O.P. and Ortwerth, B.J. Purification and properties of a protein from bovine lens which inhibits trypsin and endogenous lens proteinases.  Exp. Eye Res. 36: 363-379, 1983.

 

12.              Srivastava, O.P. and Ortwerth, B.J.  Age-related and distributional changes in the trypsin inhibitor activity of bovine lens.  Exp. Eye Res. 36: 695-709, 1983.

 

13.              Srivastava, O.P. and Ortwerth, B.J.  Isolation and characterization of a 25 K serine- proteinase from bovine lens cortex.  Exp. Eye Res. 37: 597-612, 1983.

 

14.              Srivastava, O.P., Steel, M.I. and Torres-Pinedo, R.  Maturational changes in terminal glycosylation of small intestinal microvillar proteins in rat.  Biochem. Biophys. Acta. 914: 143-151, 1987.

 

15.              Srivastava, O.P.  Characterization of a highly purified membrane proteinase from bovine lens.  Exp. Eye Res. 46: 269-283, 1988.

 

16.              Srivastava, O.P.  Age-related increase in concentration and aggregation of degraded polypeptides in human lenses.  Exp. Eye Res. 47: 525-543, 1988.

 

17.              Srivastava, O.P. and Srivastava, K.  Human lens membrane proteinase: purification and age-related distributional changes in the water soluble and insoluble protein fractions. Exp. Eye Res. 48: 161-175, 1989.

 

18.              Srivastava, O.P. and Ortwerth, B.J.  The effects of aging and cataract formation on the trypsin inhibitor activity of human lens.  Exp. Eye Res. 48: 25-36, 1989.

 

19.              Srivastava, O.P., Srivastava, K. and Shukla, S.E.  Characterization of a 66 kDa surface glycoprotein of the human corneal endothelium.  Invest. Ophthalmol. Vis. Sci. 31: 1982-1993, 1990.

 

20.              Srivastava, O.P., McEntire, J.E. and Srivastava, K.  Identification of a 9 kDa-g-crystallin fragment in human lenses.  Exp. Eye Res. 54: 893-901, 1992.

 

21.              Srivastava, O.P., Srivastava, K and Silney, K.  Identification of gs-crystallin fragments in human lenses.  Exp. Eye Res. 56: 367-369, 1993 .

 

22.              Srivastava, O.P., Srivastava, K. and Silney, C.  Identification of origin of two polypeptides of 4 and 5 kDa isolated from human lenses.  Invest. Ophthalmol. Vis. Sci., 35, 207-214, 1994.

 

23.              Srivastava, O.P., Srivastava, K. and Silney, C.  Covalent modification at the C-terminal end of a 9kDa-gD-crystallin fragment in human lenses.  Exp. Eye Res. 58: 595-604, 1994.

 

24.       Srivastava OP, Srivastava K, Silney C. Levels of crystallin fragments and identification of their origin in water soluble high molecular weight (HMW) proteins of human lenses. Curr Eye Res. 5:511-520,1996.

 

25.              Srivastava, O.P. and Srivastava, K.  Characterization of three isoforms of a 9 kDa    gD-crystallin fragment isolated from human lenses.  Exp. Eye Res. 62: 593-604, 1996.

26.       Srivastava O.P. and Srivastava K., Purification of g-crystallin from human lenses by acetone solubilization method. Curr. Eye Res., 17: 1074-1081, 1998 

 

27.       Srivastava O.P. and Srivastava K., Degradation of gD- and gs -crystallins in human lenses, Biochem. Biophys. Res. Commun. 253; 288-294,1998.

 

28.       Srivastava O.P. and Srivastava K. Age-related degradation of bA3/A1-crystallin n human lenses, Biochem. Biophys. Res. Commun. 258, 632-638, 1999

 

29.       Srivastava O.P., and Srivastava K. cAMP-Dependent Phosphorylation of big-h3 Protein in Human Corneal Endothelial Cells. Curr. Eye Res.19: 348-357, 1999 

 

30.       Srivastava O.P. and Srivastava K., Characterization of a sodium deoxycholate-   activatable proteinase activity associated with bA3/A1-crystallin of human lenses. Biochem. Biophys. Acta.1434: 331-346, 1999.

 

31.       Srivastava O.P. and Srivastava K., bB2-crystallin undergoes extensive truncation during aging in human lenses, Biochem. Biophys. Res. Commun.301:44-49, 2003.

 

32.       Srivastava O.P. and Srivastava K. Existence of deamidated aB-crystallin fragments in normal and cataractous human lenses, Mol. Vision, 9:110-118, 2003.

 

33.       Gupta R., and Srivastava O.P., Effect of deamidation of Asparagine 146 modulates functional and structural properties of human lens aB-crystallin, Invest. Ophthalmol. Vis. Sci. 45:206-214, 2004.

 

34.       Srivastava O. P. and Srivastava K. Cross-linking of human lens 9 kDa gD-crystallin fragment in vitro and in vivo, Mol. Vision, 9:644, 2003.

 

35.       Srivastava O. P., Kirk M. C. and Srivastava K. Characterization of covalent multimers of crystallins in aging human lenses J. Biol. Chem., 279:10901-10909, 2004.

36.       Harrington V., McCall S., Huynh S. and Srivastava O.P. Characterization of crystallin species present in water soluble-high molecular weight- and water insoluble-protein fractions of aging and cataractous human lenses Mol. Vis. 10:476-489,2004.

37.       Gupta R., and Srivastava O. P., Deamidation affects structural and functional properties of aA-crystallin and its oligomerization with aB-crystallin, J. Biol. Chem. 43:44258-44269, 2004.

 

38.       Gupta R., Srivastava K and Srivastava O.P. Structural and stability properties ofr human bA3-crystallin, Biochemistry, 45:9964-9978, 2006.

 

39.       Srivastava O.P., Chandrasekaran, D. and Pfister R., Molecular changes in selected proteins in human keratoconus corneas compared to normal corneas,  Mol. Vis, 12: 1615-1625, 2006.

        

40.       Harrington V. and Srivastava O.P. Proteomics of crystallin species present in water insoluble proteins of normal and cataractous human lenses, Mol Vis. 13:1680-94, 2007,

41.       Mafia K., Gupta G., Kirk M., Wilson L., Srivastava O.P. and Barnes S., UV-A-induced structural and functional changes in human lens deamidated aB-crystallin, Mol. Vis. 14:234-248, 2008.

42.       Chaves J.M., Srivastava K., Gupta R. and Srivastava O. P., Effects   of deletion of N- and C-terminal domains on human lens deamidated alpha A-crystallin Biochemistry, 47:10069-10083, 2008.

43.       Barnes A.S., Shonsey E.M., Eliuk S. M., Stella D., Barrett K., Srivastava O.P., Kim H., and Renfrow M.B., High-resolution mass spectrometry analysis of protein oxidation and resultant loss of function, Biochem. Soc. Trans., 36:1037-1044, 2008.

44.       Srivastava K., Chaves J. M, Srivastava O.P. and Kirk M. Crystallin complexes exist in the water soluble high molecular protein fractions of aging normal and cataractous human lenses Exp. Eye Res, 87:356-366, 2008.

45.       Srivastava O.P., Srivastava K. and Chaves J.M., Characterization of bA3-crystallin-assoocaited proteinase isolated from the a-crystallin fraction of human, Mol. Vis. 14:1872-1885, 2008. 

46.       Gupta R, and Srivastava OP. Identification of interaction sites between human  bA3- and aA/aB crystallins by mammalian two-hybrid- and acceptor photo-bleaching methods. J Biol Chem 2009 (Accepted pending minor revision).

47.       Srivastava K., Gupta R., Chaves J.M. and Srivastava O.P., Truncated Human βB1-Crystallin Show Altered Structural Properties and Interaction with Human βA3-Crystallin (In Press, Biochemistry, 2009)

48.       Asomugha C., Gupta R., and Srivastava O.P., In situ identification of post-translational modifications of cortical and nuclear crystallins in human lens (Submitted to Exp. Eye Res., 2009)

 

 

PAPERS PRESENTED IN NATIONAL AND INTERNATIONAL CONFERENCES:

 

1.                  Srivastava, O.P. and van Huystee, R.B.  Interrelationship among peroxidase, IAA oxidase and polypenol oxidase released in the suspension medium by peanut cells. Plant Physiol. (Suppl.) 49: 31, 1972.

 

2.                  Srivastava, O.P. and van Huystee, R.B.  Effect of splitting holoenzyme into heme and apoenzyme on peroxidase, IAA oxidase and polypenol oxidase.  Can. SOC. Plant Physiol. (Eastern meeting 1973.)

 

3.                  Srivastava, O.P. and van Huystee, R.B.  Two peroxidase isozymes from peanut cell culture filtrate.  Can. Soc. Plant Physiol. (Eastern meeting 1974.)

 

4.                  Srivastava, O.P. and Ortwerth, B.J.  Purification and properties of Phacolysin B2, a trypsin-like proteinase from bovine lens.  Int. Cong. of Eye Res., New York, USA 1980.

 

5.                  Srivastava, O.P. and Ortwerth, B.J.  Purification and properties of Phacolysin B2, a trypsin-like proteinase from bovine lens cortex.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 20: 169, 1981.

 

6.                  Srivastava, O.P. and Ortwerth, B.J.  Purification and characterization of Phacolysin B1 and B2, two trypsin-like proteinases from bovine lens cortex.  Am. Soc. Biolog. Chemists Ann. Meeting, 1981.

 

7.                  Srivastava, O.P. and Ortwerth, B.J.  Age-related changes in the proteinase and trypsin inhibitor activities of human and bovine lenses.  Invest. Ophthalmol. Vis. Sci. (Suppl.)22: 34, 1982.

 

8.                  Srivastava, O.P., Kraybill, W.G. and Tyrer, H.N.  Flow cytometric-detection of tumor specific antigens binding cells in cancer patients.  Missouri Acad. Sci., 1983.

 

9.                  Srivastava, O.P. and Kraybill, W.G. Flow-cytometric analysis of tumor antigen binding cells in cancer patients.  Ann. Meeting Am. College Surg., Missouri Chapter, 1983.

 

10.              Srivastava, O.P., Kraybill, W.G. and Tyrer, H.W.  Determination of tumor antigen binding cells in breast cancer patients using flow cytoflurometry (FCM).  Am. Soc. Biolog. Chemists, Ann. Meeting, 1984.

 

11.              Srivastava, O.P.  Isolation and characterization of a membrane-associated proteinase from bovine lens. Invest. Ophthalmol. Vis. Sci. (Suppl.) 27: 287, 1986.

 

12.              Srivastava, O.P.  Increased association of degraded polypeptides in water insoluble proteins in aging human lenses.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 28: 151, 1987.

 

13.              Srivastava, K. and Srivastava, O.P.  Age-related distributional change in a trypsin inhibitor protein of human lenses.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 28: 388, 1987.

 

14.              Srivastava, O.P. and Srivastava, K.  Proteinase activity and degraded polypeptides in normal and cataractous human lenses.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 29: 185,1988.

 

15.              Srivastava, O.P. and Srivastava, K.  Human lens membrane proteinase: Purification and age-related changes. 8th Int. Cong. Eye Res., 1988.

 

16.              Srivastava, K. and Srivastava, O.P.  Comparative levels of degraded polypeptides in cataractous and normal lenses.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 30: 336, 1989.

 

17.              Srivastava, O.P. and Srivastava, K.  Characterization of a 66 kDa surface glycoprotein of the human corneal endothelial cell.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 30: 458, 1989.

 

18.              Srivastava, K. and Srivastava, O.P.  Comparative levels of degraded polypeptides in cataractous and normal lenses.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 30: 336, 1989.

 

19.              Srivastava, O.P. and Srivastava, K.  Cyclic AMP-dependent phosphorylation of a major 66 kDa protein of the human corneal endothelium.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 31: 2325, 1990.

 

20.              Srivastava, K. and Srivastava, O.P.  Age-related insolubilization of a 9-kDa degraded polypeptide in human lenses.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 31: 808, 1990.

 

21.              Srivastava, O.P.  Cross-linking of a 9-kDa human lens polypeptide per se and with crystallins in vitro: A potential factor during cross-linking of proteins in vivo.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 32: 1060, 1991.

 

22.              Srivastava, K. and Srivastava, O.P.  Characterization of 5.5-kDa and 9 kDa- polypeptide isolated from human lens.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 32: 429, 1991.

 

23.              Srivastava, K. and Srivastava, O.P. and Silney C.  Apparent modification of a 9 kDa polypeptide during aging in human lenses.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 33: 867, 1992.

 

24.              Srivastava, O.P. and McEntire, J.E.  Human lens membrane proteinase: partial N-terminal amino acid sequence and activation.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 32: 869, 1992.

 

26.       Srivastava, O.P., Srivastava, K. and Silney K.  Characterization of crystallin fragments (degraded polypeptides) of water soluble (WS)-high molecular weight (HMW) proteins in aging human lenses.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 34: 1401, 1993.

 

27.       Srivastava, O.P. and Srivastava, K. Hejtmancik, J.F. and Hope, T. Age-related degradative pathways of human lens bA3/A1 and gD-crystallins are programmed.  Invest. Ophthalmol. Vis. Sci.  (Suppl.) 35: 280, 1994.

 

28.       Srivastava O.P. and Srivastava K., Degradation of gD-crystallin during aging and cataractogenesis, Exp. Eye Res. (Suppl.): S-23, 1994.

 

29.       Srivastava O.P. and Srivastava K., Identification and characterization of post-translationally derived isomeric forms of a 9-kDa gD-crystallin fragment, Invest. Ophthalmol. Vis. Sci. (Suppl), 36: 4049, 1995.

 

30.       Srivastava, K. and Srivastava, O.P..  Characterization of properties of a 66-kDa protein from human corneal endothelial cells.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 37: 413, 1996.

 

31.       Srivastava, O.P. and Srivastava, K..  Purification and characterization of a sodium deoxycholate activable-proteinase activity possibly associated with bA3/A1-crystallin, from human lenses.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 37: 1949, 1996.

 

32.       Srivastava, O.P. and Srivastava, K.  Purification of g-crystallin is an acetone solubilization method.  Invest. Ophthalmol Vis. Sci. (Suppl.) 38: 1374, 1997.

 

33.       Tu, M. and Srivastava, O.P.  Purification and characterization of a sodium deoxycholate-activable proteinase from bovine lenses.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 38: 1383, 1997.

 

34.       Srivastava O.P. and Srivastava K. and Hejtmancik, J.F. Association of proteinase activity with mouse recombinant bA3/A1-crystallin. Invest. Ophthalmol. Vis. Sci. (Suppl.), 39, 4049, 1998.

 

35.       Acharya H.R. and Srivastava, O.P. g-crystallin degradation and association of its fragments with water-soluble high molecular weight (WS-HMW)-proteins. Invest. Ophthalmol. Vis. Sci.(Suppl.), 39, 4896, 1998.

 

36.       Srivastava O.P. and Srivastava  K., Cyclic AMP (cAMP)-dependent phosphorylation of surface proteins from human and rabbit corneal endothelia. Invest. Ophthalmol. Vis. Sci.(Suppl.), 39, 2433, 1998.

 

37.       Srivastava O.P. and Srivastava K., Human lens a-crystallin fraction contains an Arg-bond hydrolyzing proteinase. Invest. Ophthalmol. Vis. Sci. (Suppl.), 40, 1593, 1999.

 

38.       Srivastava K. and Srivastava O.P. Identification of in vivo cleavage sites in human lens bA3/A1- and bB2-crystallins. Invest. Ophthalmol. Vis. Sci. (Suppl.), 40, 1594, 1999.

 

39.       Sisneros, G., Srivastava, O.P. and Srivastava, K.  Cross-linking of human lens 9kDa γD-crystallin fragment in vitro:  A potential factor during cross-linking of proteins in vivo.  Invest. Ophthalmol. Vis. Sci. (Suppl) 41: 3116, 2000.

 

40.       Srivastava, O.P., and Srivastava, O.P.  An association of a single Arg-bond hydrolyzing proteinase with different crystalline fractions and its inhibition by α-crystallin in human lenses.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 41: 3118, 2000.

 

41.       Brockway, L. and Srivastava, O.P.  Analysis of big h3 protein in bovine corneal epithelium and endothelium.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 41: 1378, 2000.

 

42.       Srivastava, O.P., Bansal, O., Kontzen, P. Srivastava, K. and Pfister, R.  Expression of big h3 protein in normal, Fuchs' dystrophic and keratoconus corneas.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 41: 1425, 2000.

 

43.       Srivastava, O.P., Srivastava, K. and Thompson J. Characterization of crystallin fragments from normal and cataractous human lenses following isolation by a TCA solubilization method. Invest. Ophthalmol. Vis. Sci. (Suppl.) 42: 1593, 2001.

 

44.       Srivastava, K and   Srivastava, O.P., Determination of in vitro proteolysis of g-crystallins by human lens Arg-bond hydrolyzing proteinase,  invest. Ophthalmol. Vis. Sci. (Suppl.) 42: 4717, 2001.

 

45.       Harrington V., and Srivastava O.P., Age-related degradation in a-crystallin in human lenses, Invest. Ophthalmol. Vis. Sci. (Suppl.) 43: 474, 2002

 

46.       Chandrasekaran D. and Srivastava O.P., Determination of molecular changes in selected epithelial proteins in human keratoconus corneas compared to normal corneas. Invest. Ophthalmol. Vis. Sci. (Suppl.) 43: 1664, 2002

 

47.       Huynh S., McCall S. and Srivastava O.P., Cataract-specific degradation of alpha crystallin fragments in human lenses, Invest. Ophthalmol. Vis. Sci. (Suppl.) 43: 3558, 2002.

 

48.       Srivastava, O.P. and Srivastava, K.,  Alpha crystallin fraction from human lenses contains a proteinase, Invest. Ophthalmol. Vis. Sci. (Suppl.) 43: 4651, 2002.

 

49.       Srivastava, K. and Srivastava, O.P., Determination of modifications in three isoforms of a 9 kDa gamma D-crystallin fragment isolated from human lenses, Invest. Ophthalmol. Vis. Sci. (Suppl.) 43: 4659, 2002.

 

50.                   Srivastava O.P. and Srivastava K., Characterization of covalent multimers of crystallins in water insoluble fraction of human lenses. Invest. Ophthalmol. Vis. Sci. (Suppl.) 44: 2344, 2003.

 

51.                                           Gupta R. and Srivastava O.P. Effect of sit-directed mutagenesis (deamidation of N78, N146, and N78/N146) on chaperone activity of aB-crystallin. Invest. Ophthalmol. Vis. Sci. (Suppl.) 44: 2362, 2003.

 

52.                                           Harrington V. and Srivastava O.P., Localization of a-crystallin in aging normal and cataractous lenses. Invest. Ophthalmol. Vis. Sci. (Suppl.) 44: 2364, 2003.

 

53.       Harrington V., and Srivastava O.P. Analysis of crystallin species present in water insoluble-urea insoluble protein fractions in normal and age-matched cataractous human lenses,  Invest. Ophthalmol. Vis. Sci. (Suppl.) 45: 1682, 2004.

 

54.                                           Srivastava O.P. and Srivastava K., Association of proteinase activity with beta A3/A1-crystallin, Invest. Ophthalmol. Vis. Sci. (Suppl.) 45:3964, 2004.

 

55.           Gupta R., and Srivastava O.P., Deamidation alters structural and functional properties of aA-crystallin and oligomerization with aB-crystallin, Invest. Ophthalmol. Vis. Sci. (Suppl.) 45: 3979, 2004.

 

56.       Srivastava O.P., and K. Srivastava, Inhibition of a lens endogenous proteinase by a-crystallin, Abstract no. 3875, Association for Research in Vision and Ophthalmology, Ft. Lauderdale, Florida, 2005.

           

57.       Gupta R., Srivastava K., and Srivastava O.P., Potential role of N-terminal extension and individual motifs (I to IV) on structural stability of human  bA3/A1-crystallin, Abstract no. 3877, Association for Research in Vision and Ophthalmology, Ft. Lauderdale, Florida, 2005.

 

58.       Gupta R., Srivastava K. and  Srivastava O.P. Folding/refolding properties of N-terminal extension and domains of human βA3/A1 Crystallin. Association for Research in Vision and Ophthalmology meeting, Ft. Lauderdale, Florida, 2006.

 

59        Chaves J.M., Asomugha  C., Srivastava K., Gupta R. and Srivastava O.P. Characterization of truncated/deamidated mutants of human alpha A and alpha B crystallins. Association for Research in Vision and Ophthalmology meeting, Ft. Lauderdale, Florida, 2006.

 

60.       Srivastava O.P., Srivastava K., and Gupta R., Associaition of an Arg-bond hydrolyzing activity with human bA3/A1-CRYSTALLIN, Abstract no. 4875, Association for Research in Vision and Ophthalmology, Ft. Lauderdale, Florida, 2006.

 

61.              Srivastava O.P., Srivastava K. and Gupta R., Structural and stability properties of truncated Human βA3/A1 Crystallin. XVII International Congress of Eye Research Meeting, Buenos Aires, Argentina, 2006.

62.       Stella D. R., Gupta R., Srivastava O.P. and Barnes S.,  Human bA3-crystallin modification after UV induction,  Sixth  Annual Purdue-UAB Botanicals Center Symposium, Birmingham, Alabama, 2006.

63.       Mafia K,  Gupta R., Kirk M., Srivastava O.P. and Barnes S. Effects of UVA light on human lens deamidated aB-crystallin. Sixth  Annual Purdue-UAB Botanicals Center Symposium, Birmingham, Alabama, 2006.

64.              Barnes  S., Wang C-C, Srivastava O, Mass  spectrometry profiling of the lens proteins of ICR/f rat, 7th Annual Meeting and Symposium of Botanical Research Center, Purdue University, 2007.

65.      Srivastava O.P., Mafia K, Gupta R., Kirk M., and Barnes S., Effects of UVA-irridiation on human lens native and deamidated aB-crystallin, Annual meeting and Symposium of Botanical Research Center, Purdue University, 2007

 

66.       Asomugha, C.O., Gupta R. Chaves J.M., Srivastava K., and Srivastava O.P., Effect of truncations of N and C-terminal domains on deamidated human alpha B crystallin, Abstract no. 2037,  Association for Research in Vision and Ophthalmology, Ft. Lauderdale, Florida, 2007.

 

67.       Chaves J.M., Srivastava K., Asomugha C., Gupta R., and Srivastava O.P., Effect of truncations of N and C-terminal domains on deamidated human alpha A crystallin, Abstract no. 2038,  Association for Research in Vision and Ophthalmology, Ft. Lauderdale, Florida, 2007.

 

68.       Srivastava O.P., Srivastava K., Gupta R., Is and Arg-bond hydrolyzing proteinase activity associated with human lens bA3/A1-crystallin? Mini symposium

“Proteases in lens development and disease”, Abstract no. 2861, Association for Research in Vision and Ophthalmology, Ft. Lauderdale, Florida, 2007.

 

69.      Gupta R and Srivastava OP, Interactions between motifs of human βA3/A1 crystallin and aA and aB-crystallins, Association for Research in Vision and Ophthalmology meeting, Ft. Lauderdale, Florida, 2008.

 

70.       Srivastava K., Chaves J.M. and Srivastava O.P., Characterization of crystallin complexes in high molecular weight proteins of aging normal and cataractous human lenses by blue-native gel electrophoresis, Association for Research in Vision and Ophthalmology meeting, Ft. Lauderdale, Florida, 2008.

       

71.       Asomugha C.O., Gupta R., and Srivastava O.P., In Situ regional distribution of crystallins in aging human lens using laser capture microdissection, Association for Research in Vision and Ophthalmology meeting, Ft. Lauderdale, Florida, 2008.

    

72.       Chaves J.M., Gupta R., Srivastava K. and Srivastava O.P., Interaction of human aA-crystallin with phakinin and filensin, Association for Research in Vision and Ophthalmology meeting, Ft. Lauderdale, Florida, 2008.